Bovine Leptin Lep ELISA Kit size: 96 Tests

Introduction

The Bovine Leptin Lep ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) designed to quantitatively measure bovine leptin in serum, plasma, or other biological samples. Leptin, a hormone predominantly produced by adipocytes, plays a significant role in regulating energy balance, body weight, and reproductive functions in cattle. The assay utilizes a highly specific antibody to detect bovine leptin and is optimized for reliable and precise results.

Kit Components

The 96-test ELISA Kit includes the following components necessary for performing the assay:

1. Coated Microplate: A 96-well microplate pre-coated with anti-bovine leptin antibody. This serves as the solid phase where the target bovine leptin will be captured during the assay.
2. Standards: A set of bovine leptin standards at known concentrations, which is used to generate a standard curve for quantifying leptin levels in the samples.
3. Sample Diluent: A buffer used for diluting the samples to ensure proper measurement within the kit's detection range.
4. Conjugate (Horseradish Peroxidase, HRP): A bovine leptin-specific antibody conjugated to HRP, which catalyzes a colorimetric reaction used to detect leptin.
5. Substrate Solution: A substrate for HRP that produces a color change proportional to the amount of leptin present in the sample.
6. Stop Solution: A solution used to stop the colorimetric reaction, ensuring a stable and measurable result.
7. Wash Buffer: A buffer used to wash the microplate between assay steps, removing unbound materials to reduce background noise and ensure specific binding.

Assay Procedure

1. Plate Preparation: The provided microplate is ready for use. Ensure the wells are appropriately labeled for the standards and samples.
2. Sample and Standard Addition: Add standards and samples to the wells. The leptin in the samples will bind to the pre-coated anti-bovine leptin antibody.
3. Conjugate Addition: After a brief incubation, add the HRP-conjugated antibody, which binds specifically to leptin, forming a complex.
4. Substrate Reaction: Add the substrate solution, which reacts with the HRP enzyme, producing a color change.
5. Stopping the Reaction: Once the desired color intensity is achieved, add the stop solution to terminate the reaction.
6. Measurement: Measure the optical density (OD) at 450 nm using a microplate reader.

Key Features

• Sensitivity: The kit offers high sensitivity, enabling accurate measurement of low levels of leptin in biological samples.
• Range: The assay covers a wide concentration range, allowing for both low and high leptin levels to be quantified effectively.
• Specificity: The monoclonal antibody used in the assay is highly specific for bovine leptin, ensuring minimal cross-reactivity with other molecules.
• Precision: The kit provides reproducible results, making it suitable for both research and clinical applications.
• Time-efficient: The assay can be completed in approximately 2-3 hours, including the incubation times and measurement steps.

Applications

• Research on Bovine Metabolism: Leptin is a critical marker in understanding the energy balance, fat storage, and metabolic processes in cattle. This ELISA kit helps in the study of nutrition, body condition scoring, and obesity in livestock.
• Endocrine Research: It is used in studies of hormone regulation, including its role in reproduction, growth, and appetite control in cattle.
• Veterinary Diagnostics: The assay is used in veterinary practices to monitor leptin levels in cattle as part of health assessments, particularly in relation to metabolic diseases.

Handling and Storage

• Storage: Store the kit components at 2-8°C to maintain the integrity of the reagents and ensure accurate results.
• Shelf Life: The kit is stable for 12 months from the date of manufacture when stored properly.
• Sample Storage: Samples should be stored at -20°C for long-term storage and thawed before use.

Conclusion

The Bovine Leptin Lep ELISA Kit (96 Tests) provides an efficient and reliable method for measuring leptin levels in bovine samples. With its high sensitivity, specificity, and reproducibility, the kit is suitable for a wide range of applications, including research on metabolic diseases, reproductive health, and veterinary diagnostics. By quantifying leptin in cattle, researchers and clinicians can gain insights into key physiological processes and better understand the role of leptin in bovine health and productivity.